Murine Lymphoma and PBMC Isolation

Murine L5178Y-R lymphoma cells (ATCC CRL-1722) and PBMC were used in this study. PBMC were obtained from a 50 mL to 60 mL blood sample from a healthy donor, using Ficoll-Paque PLUS (GE Healthcare Life Sciences, Pittsburgh, PA, USA) and following supplier’s instructions. L5178Y-R cells and PBMC were maintained in RPMI 1640 culture medium (Life Technologies, Grand Island, NY, USA), supplemented with 10% heat-inactivated fetal bovine serum (FBS; Life Technologies) and 1% antibiotic/antifungal solution (Life Technologies) (referred as complete 1640 medium) at 37 °C in an atmosphere of 5% CO₂ in air [17 (link)].

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Rodríguez-Garza N.E., Quintanilla-Licea R., Romo-Sáenz C.I., Elizondo-Luevano J.H., Tamez-Guerra P., Rodríguez-Padilla C, & Gomez-Flores R. (2023). In Vitro Biological Activity and Lymphoma Cell Growth Inhibition by Selected Mexican Medicinal Plants. Life, 13(4), 958.

Publication 2023

Murine L5178Y-R lymphoma cells Ficoll-Paque PLUS RPMI 1640 culture medium antibiotic/antifungal solution

Antibiotic Antifungal Atmosphere Cells Donor blood Ficoll Lymphoma Murine

Corresponding Organization : Universidad Autónoma de Nuevo León

Other organizations : Instituto de Investigación Biomédica de Salamanca, Universidad de Salamanca

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Variable analysis

independent variables

Cell type (Murine L5178Y-R lymphoma cells and PBMC)

dependent variables

Not explicitly mentioned

control variables

Culture medium (RPMI 1640 with 10% FBS and 1% antibiotic/antifungal solution)
Incubation conditions (37 °C, 5% CO2)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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