Intact Glycopeptides Analysis Protocol

One microgram of glycopeptides was separated on an EASY-nLC™ 1200 instrument (Thermo Fisher Scientific, Germany) with the use of Acclaim PepMap100 precolumn (2 cm, 75 µm i.d., 3 µm) and Acclaim PepMap100 separating column (50 cm, 75 µm i.d., 3 µm) operating at 300 nL/min. The mobile phase A (0.1% FA) and mobile phase B (0.1% FA / 80% ACN) with a flow rate of 200 nL/min. The parameters used for intact glycopeptides analysis were the same as previously described [22 (link), 23 (link)].

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Yu H., Li M., Shu J., Dang L., Wu X., Wang Y., Wang X., Chang X., Bao X., Zhu B., Ren X., Chen W, & Li Y. (2023). Characterization of aberrant glycosylation associated with osteoarthritis based on integrated glycomics methods. Arthritis Research & Therapy, 25, 102.

Publication 2023

EASY-nLC™ 1200 instrument Acclaim PepMap100 precolumn Acclaim PepMap100 separating column

Glycopeptides

Corresponding Organization :

Other organizations : Northwest University, Xi'an Honghui Hospital, Xi'an Jiaotong University, Shaanxi University of Chinese Medicine

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Variable analysis

independent variables

Separating column (50 cm, 75 µm i.d., 3 µm)
Flow rate (300 nL/min)
Mobile phase A (0.1% FA)
Mobile phase B (0.1% FA / 80% ACN)
Flow rate (200 nL/min)

dependent variables

Intact glycopeptides analysis

control variables

Precolumn (2 cm, 75 µm i.d., 3 µm)
Parameters used for intact glycopeptides analysis as previously described [22, 23]

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