Example 4
To obtain recombinant chimeric anti-FOLR1 mAbs, the expression vectors containing the mouse variable regions (VH and VL) fused to the constant regions of human IgG1 heavy chain and kappa light chain, respectively, were transiently transfected into 293E cells. The recombinant antibodies produced in the suspension of the transfected cells were purified using Protein A affinity chromatography.
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