Immortalized Fibroblast Cell Lines from Patient and Mouse

HAF were established from skin biopsies derived from patient II.5 and unrelated controls (Co1-3). MEF and adult cardiac fibroblasts (ACF) were derived from Yme1l1 mutant mice (Yme1l1^loxP/loxP) as described previously (Anand et al., 2014 (link); Wai et al., 2015 (link); Yao and Shoubridge, 1999 (link)). HAF, MEF, and ACF were cultured in high glucose Dulbecco’s modified Eagle’s medium (Gibco, Darmstadt, Germany) supplemented with 15%, 10%, and 10% fetal bovine serum (FBS, Biochrome, Berlin, Germany; Life Technologies; Carlsbad, California), respectively, and 1% penicillin-streptomycin (Sigma Aldrich; St. Louis, Missouri). Fibroblasts were immortalized by stable expression of hTERT and E7 (Zhu et al., 1998 (link)).