Antibodies Used in Cellular Assays

The following antibodies were used in this study: anti-MISP (rb)^{2 (link)} anti-MISP (ms, kind gift from Alwin Krämer)^{3 (link)}, anti-IQGAP1 (rb, Abcam 86064), anti-IQGAP1 (ms, Santa Cruz sc-376021), anti-Cdc42 (ms, Cytoskeleton, ACD03), anti-pericentrin (rb, Abcam ab4448), anti-β-actin (ms, Calbiochem JLA20), anti-α-tubulin (ms, Sigma B-5-1-2, T5168), anti-GFP (rb, Novus Biologicals NB600-308), anti-FLAG M2 (ms, Sigma F1804), anti-GST Z5 (rb, Santa Cruz sc-459), anti-EB1 (ms, BD Biosciences 610535), anti-pAkt (S473, ms, CST #587F11), anti-Akt (rb, CST #9272), anti-p150^glued (ms, BD Biosciences 612709). Secondary antibodies: Alexa 488/568/647-coupled anti-rabbit/mouse IgG (Molecular probes).

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Vodicska B., Cerikan B., Schiebel E, & Hoffmann I. (2018). MISP regulates the IQGAP1/Cdc42 complex to collectively orchestrate spindle orientation and mitotic progression. Scientific Reports, 8, 6330.

Publication 2018

NB600-308

Actin Alexa 568 Anti igg Antibodies Biologicals Cdc42 Cytoskeleton Molecular probes Mouse Novus Pericentrin Rabbit α tubulin

Corresponding Organization :

Other organizations : German Cancer Research Center, DKFZ-ZMBH Alliance

Top 5 similar protocols

Variable analysis

independent variables

Anti-MISP (rb)
Anti-MISP (ms, kind gift from Alwin Krämer)
Anti-IQGAP1 (rb, Abcam 86064)
Anti-IQGAP1 (ms, Santa Cruz sc-376021)
Anti-Cdc42 (ms, Cytoskeleton, ACD03)
Anti-pericentrin (rb, Abcam ab4448)
Anti-β-actin (ms, Calbiochem JLA20)
Anti-α-tubulin (ms, Sigma B-5-1-2, T5168)
Anti-GFP (rb, Novus Biologicals NB600-308)
Anti-FLAG M2 (ms, Sigma F1804)
Anti-GST Z5 (rb, Santa Cruz sc-459)
Anti-EB1 (ms, BD Biosciences 610535)
Anti-pAkt (S473, ms, CST #587F11)
Anti-Akt (rb, CST #9272)
Anti-p150glued (ms, BD Biosciences 612709)
Alexa 488/568/647-coupled anti-rabbit/mouse IgG (Molecular probes)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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