Whole-Genome Sequencing of S. haemolyticus

Whole-genome sequencing of the two strains of S. haemolyticus were performed by Genewiz Biotechnology Co. Ltd. (Suzhou, China) using paired-end sequencing with Novaseq 6000 (Illumina, 2×150 bp paired-end reads) and long sequencing with PacBio Sequel IIe (Pacific Biosciences, 10–15 Kb insert whole-genome shotgun libraries). PacBio reads were assembled using the Hifiasm software15 (link) version 0.13-r308 and Canu16 (link) version 1.7. The genome assembly was then polished using Pilon software version 1.2217 (link) using Illumina reads. The assembled genomes of the two strains of S. haemolyticus were submitted to the National Center for Biotechnology Information (NCBI) GenBank database and annotated using the NCBI Prokaryotic Annotation Pipeline (PGAP).

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Liu Z., Wang L., Sun J., Zhang Q., Peng Y., Tang S., Zhang L., Li X., Yu Z, & Zhang T. (2024). Whole Genome Sequence Analysis of Two Oxacillin-Resistant and mecA-Positive Strains of Staphylococcus haemolyticus Isolated from Ear Swab Samples of Patients with Otitis Media. Infection and Drug Resistance, 17, 1291-1301.

Publication 2024

Novaseq 6000 PacBio Sequel IIe

Corresponding Organization :

Other organizations : Zhuhai People's Hospital, Jinan University, First Affiliated Hospital of Jinan University

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Variable analysis

independent variables

Whole-genome sequencing of the two strains of S. haemolyticus

dependent variables

Genome assembly

control variables

Whole-genome sequencing was performed using paired-end sequencing with Novaseq 6000 (Illumina, 2×150 bp paired-end reads) and long sequencing with PacBio Sequel IIe (Pacific Biosciences, 10–15 Kb insert whole-genome shotgun libraries)
PacBio reads were assembled using the Hifiasm software version 0.13-r308 and Canu version 1.7
The genome assembly was then polished using Pilon software version 1.22 using Illumina reads
The assembled genomes of the two strains of S. haemolyticus were submitted to the National Center for Biotechnology Information (NCBI) GenBank database and annotated using the NCBI Prokaryotic Annotation Pipeline (PGAP)

Annotations

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