Crystallization Screening of OsJAC1 Domains

OsJAC1 and its two individual domains were prepared as previously described in Huwa et al. (2021) [25 (link)]. The initial crystallization screening conditions of the two domains that led to successful crystallizations, are listed in Table 1. All solutions were prepared or diluted with ddH₂O. Laminaribiose and 1,4-β-d-galactobiose were purchased from Megazyme (Bray, Ireland). Dithiothreitol (DTT) was purchased from Fisher Chemical (Schwerte, Germany). Rabbit blood in Alsever’s solution was purchased from Fiebig-Nährstofftechnik GbR (Idstein-Niederauroff, Germany). Screening sets for protein crystallization were purchased from Merck (Darmstadt, Germany) and Qiagen (Hilden, Germany).