In order to investigate the intravitreal toxicity of the PaPn11 and PaPn13
peptides, the animals were anesthetized via intraperitoneal injection of 90mg/kg
ketamine (Dopalen; Ceva, Brazil) plus 10 mg/kg xylazine hydrochloride (Anasedan;
Ceva, Brazil). Sequentially, the right eyes were anesthetized using one drop of
0.5% (w/v) proxymetacaine hydrochloride (Anestalcon; Alcon, Brazil). For
intravitreal injections, the animals were divided into three main groups: PnPa11
(n = 20), PnPa13 (n = 20) and vehicle (saline) (n = 4). The groups that received
the synthetic peptide were subdivided according to the intravitreal
concentration of the peptides administrated (0.50; 1.25; 2.50; 3.75 and 5.00
µg/mL) (n = 4). The left eyes of all animals were kept intact.
A 30-gauge needle attached to a syringe was inserted ∼2 mm to the limbus. Besides
that, the needle was held in place for 30seconds to prevent it from escaping the
application site. The volume of intravitreal injection was set to 10 µL [28 (link)]. Each concentration was calculated
based on the dilution that occurs in the vitreous humor (for adult rats, the
vitreous volume is about 50 μL) [29 ].
peptides, the animals were anesthetized via intraperitoneal injection of 90mg/kg
ketamine (Dopalen; Ceva, Brazil) plus 10 mg/kg xylazine hydrochloride (Anasedan;
Ceva, Brazil). Sequentially, the right eyes were anesthetized using one drop of
0.5% (w/v) proxymetacaine hydrochloride (Anestalcon; Alcon, Brazil). For
intravitreal injections, the animals were divided into three main groups: PnPa11
(n = 20), PnPa13 (n = 20) and vehicle (saline) (n = 4). The groups that received
the synthetic peptide were subdivided according to the intravitreal
concentration of the peptides administrated (0.50; 1.25; 2.50; 3.75 and 5.00
µg/mL) (n = 4). The left eyes of all animals were kept intact.
A 30-gauge needle attached to a syringe was inserted ∼2 mm to the limbus. Besides
that, the needle was held in place for 30seconds to prevent it from escaping the
application site. The volume of intravitreal injection was set to 10 µL [28 (link)]. Each concentration was calculated
based on the dilution that occurs in the vitreous humor (for adult rats, the
vitreous volume is about 50 μL) [29 ].
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