TMAO Quantification by NMR Spectroscopy

TMAO concentrations were quantified in serum samples using Nuclear Magnetic Resonance (NMR) as previously described [62 (link)]. The NMR spectra were analyzed in a Bruker Avance IVDr 600 MHz spectrometer (Bruker Biospin, Rheinstetten, Germany) and processed using the TopSpin program (Bruker Biospin, Germany). Three NMR spectra were analyzed separately for each sample. The intensity of the TMAO peaks were measured for all spectra and the concentrations in each sample were calculated with fitted calibration curves.

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Sánchez-Alcoholado L., Ordóñez R., Otero A., Plaza-Andrade I., Laborda-Illanes A., Medina J.A., Ramos-Molina B., Gómez-Millán J, & Queipo-Ortuño M.I. (2020). Gut Microbiota-Mediated Inflammation and Gut Permeability in Patients with Obesity and Colorectal Cancer. International Journal of Molecular Sciences, 21(18), 6782.

Publication 2020

TopSpin program

Nuclear magnetic resonance Serum Tmao

Corresponding Organization : Instituto de Investigación Biomédica de Málaga

Other organizations : Universidad de Málaga, Instituto Murciano de Investigación Biosanitaria

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Variable analysis

independent variables

NMR spectra analysis method (Bruker Avance IVDr 600 MHz spectrometer, TopSpin program)

dependent variables

TMAO concentrations in serum samples

control variables

Three NMR spectra analyzed separately for each sample
Intensity of TMAO peaks measured for all spectra
Concentrations calculated with fitted calibration curves

controls

No positive or negative controls explicitly mentioned

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