ChIP Analysis of PIK3IP1 Promoter

ChIP was performed as we have previously described ^{33 (link)}. The following antibodies were used to perform ChIP: anti-H3K27Me3 (Cell Signaling, Cat. No: 9733), anti-ARID1A (Santa Cruz, Cat. No: sc-32761), anti-BRG1 (Santa Cruz, Cat. No: sc-10768), anti-RNA polymerase II (Santa Cruz, Cat. No: sc-899) or anti-EZH2 (Cell Signaling, Cat. No: 5246). An isotype matched IgG was used as a negative control. ChIP DNA was analyzed by quantitative PCR against the promoter of the human PIK3IP1 gene using the following primers: Forward: 5’-CACATTGA-GCTGGTGTTTGTT-3’ and Reverse: 5’-CCATTGCCACTTCAAAGAGTTT-3’.

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Biter B.G., Aird K.M., Garipov A., Li H., Amatangelo M., Kossenkov A.V., Schultz D.C., Liu Q., Shih I.M., Conejo-Garcia J.R., Speicher D.W, & Zhang R. (2015). Targeting EZH2 methyltransferase activity in ARID1A mutated cancer cells is synthetic lethal. Nature medicine, 21(3), 231-238.

Publication 2015

anti-H3K27Me3 anti-ARID1A anti-BRG1 anti-RNA polymerase II anti-EZH2

Antibodies Arid1a Brg1 Chip Ezh2 Gene promoter Human Isotype Primers Rna polymerase ii

Corresponding Organization :

Other organizations : The Wistar Institute, Johns Hopkins University

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Variable analysis

independent variables

Anti-H3K27Me3 antibody
Anti-ARID1A antibody
Anti-BRG1 antibody
Anti-RNA polymerase II antibody
Anti-EZH2 antibody

dependent variables

Enrichment of DNA at the promoter of the human PIK3IP1 gene

control variables

Isotype matched IgG (negative control)

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