Antigen-retrieval and signal detection were performed as previously described 20 (link). The following primary Abs were used: goat polyclonal anti-NKp46 (R&D Systems), mouse anti-CD56 (clone 123C3, Dako), mouse anti-CD68 (clone KP1, Dako), mouse anti-neutrophil defensins (clone D21, Leica Biosystems), mouse anti-CD16 (clone 2H7, Leica Biosystems), rabbit anti-CD32 (clone EPR6657(2), Abcam), mouse anti-CD20 (clone L26, Dako), rabbit polyclonal anti-CD3 (Dako). The following control Abs were used: mouse IgG1 (Dako), goat IgG (R&D Systems), mouse IgG2a (Abcam) and rabbit IgG (Abcam).
Protocol detail
Immunohistochemical Profiling of Immune Cells
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Ragon Institute of MGH, MIT and Harvard
Other organizations : Ghent University, Dartmouth College, University of Duisburg-Essen, University of Minnesota
Top 5 similar protocols
Protocol cited in 2 other protocols
Variable analysis
independent variables
- Primary antibodies used: goat polyclonal anti-NKp46, mouse anti-CD56 (clone 123C3), mouse anti-CD68 (clone KP1), mouse anti-neutrophil defensins (clone D21), mouse anti-CD16 (clone 2H7), rabbit anti-CD32 (clone EPR6657(2)), mouse anti-CD20 (clone L26), rabbit polyclonal anti-CD3
- Unspecified
- Control antibodies used: mouse IgG1, goat IgG, mouse IgG2a, and rabbit IgG
- Positive controls: Not specified
- Negative controls: mouse IgG1, goat IgG, mouse IgG2a, and rabbit IgG
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!