Microstructural analysis was conducted using a Deltavision OMX SR super-resolution microscope (GE Co., Boston, Massachusetts, USA) to elucidate the particle size and distribution of the nanoemulsions. Based on the work of Liu et al. [27 (link)], 1 mL of the nanoemulsions was mixed with 20 μL of Nile red (0.1%, w/v) and then left in the dark for 30 min after vortex mixing. The stained nanoemulsion samples (5 μL) were deposited on a concave slide with a coverslip. The samples were stored at 4 °C for 12 h before observation.
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