Liver cancer TMAs composed of 110 FFPE tissue Sects. (4 μm thick) were prepared the same way as previously described by Guttà C et al. [31 (link)] TMA IHC staining was conducted based on a standard approach. Briefly, the sections were deparaffinized using xylene, rehydrated with an ethanol gradient, treated to quench peroxidase activity, blocked with 5% normal goat serum, and incubated with anti-YAP/GLUT1 (1:200) overnight at 4 °C. As a negative control, the antibody was omitted. The slides were then probed with biotin-labeled goat anti-rabbit IgG, treated with a streptavidin peroxidase solution (SABC kit, Boster, Wuhan, China), and then 3,3-diaminobenzidine (Boster, Wuhan, China) in PBS with 0.05% H2O2 was used to treat the samples for 5 min at the room temperature for color development, after which the samples were counterstained using hematoxylin.
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