Protocol detail

Synthesis and Characterization of Paclitaxel-HA Conjugates

Find Similar Protocols

Bovine casein (>98%), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were obtained from Sigma–Aldrich, Chemical Co. (St. Louis, MO, USA). Paclitaxel (>99%) and cis-diammineplatinum dichloride were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Human malignant melanoma cells (A375) were purchased from Shanghai Institutes for Biological Sciences (Chinese Academy of Sciences). All materials for cell culture were obtained from Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China. The preparation of specific-molecular-weight HA oligosaccharides (10,000 Da) was performed by using the methods mentioned in our previous study [12 (link),16 (link)]. High-molecular-weight HA was hydrolyzed by leech hyaluronidase, and the molecular weight of hydrolyzed HA was determined by high performance size exclusion chromatography. All other reagents were of analytical grade.

Free full text: Click here

Wang M., Zhang Y., Fei Z., Xie D., Zhang H., Du Q, & Jin P. (2022). Hyaluronan Oligosaccharides-Coated Paclitaxel-Casein Nanoparticles with Enhanced Stability and Antitumor Activity. Nutrients, 14(19), 3888.

Publication 2022

Bovine casein 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) N-hydroxysuccinimide (NHS) Paclitaxel

Bovine Casein Cell Chinese Human Hyaluronidase Leech Malignant melanoma N hydroxysuccinimide Oligosaccharides Paclitaxel Size exclusion chromatography

Top 5 similar protocols

Variable analysis

independent variables

Bovine casein (>98%)
1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC)
N-hydroxysuccinimide (NHS)
Paclitaxel (>99%)
Cis-diammineplatinum dichloride
Human malignant melanoma cells (A375)
High-molecular-weight HA hydrolyzed by leech hyaluronidase

dependent variables

Not explicitly mentioned

control variables

All materials for cell culture were obtained from Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!