Nuclear extracts were prepared from colon tissues. Nuclear extracts was obtained with the use of a nuclear extraction kit (Active Motif). Binding activity of nuclear extract (5 μg) to NF-κB subunit (p50, p65, c-Rel) was measured using a Transam kit, obtained from Active Motif, as previously described 43 (link).
Protocol detail
Quantifying NF-κB Activity in Colon Tissues
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Corresponding Organization :
Other organizations : Center for Innovation, National Institutes of Health, Kyoto University, National Institute of Allergy and Infectious Diseases, Tohoku University, Kindai University
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Variable analysis
independent variables
- Nuclear extract preparation method (use of a nuclear extraction kit)
- Binding activity of nuclear extract (5 μg) to NF-κB subunit (p50, p65, c-Rel)
- Amount of nuclear extract used (5 μg)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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