Comprehensive Reagents and Inhibitors Protocol

Lysotracker Red DND-99 (L7528) was from ThermoFisher Scientific. Antimycin A (A8674), DFP (379409), DMOG (D3695), SGC-GAK-1 (GAKi, SML2202), SGC-GAK-1N (GAKc, SML2203) and QVD-OPh (SML0063) were from Sigma Aldrich. Bafilomycin A1 (BML-CM110), CCCP (BML-CM124) were from Enzo Life Sciences. Enzastaurin (S1055), oligomycin A (S1478), and sotrastaurin (S2791) were from Selleckchem. MRT68921 (1190379-70-4) and VPS34-IN1 (1383716-33-3) were from Cayman Chemical. IVAP1966 (12 g) and IVAP1966 (12i) were gratefully received from the lab of Prof. Piet Herdewijn²⁹. HY-19764 was gratefully received from the structural genomics consortium^{30 (link)}. Bradford reagent dye (#5000006) was from Bio-Rad. 1,4-dithiothreitol (DTT, #441496P) was from VWR. Complete EDTA-free protease inhibitors (#05056489001) and phosphatase inhibitors (#04906837001) were from Roche.

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Munson M.J., Mathai B.J., Ng M.Y., Trachsel-Moncho L., de la Ballina L.R., Schultz S.W., Aman Y., Lystad A.H., Singh S., Singh S., Wesche J., Fang E.F, & Simonsen A. (2021). GAK and PRKCD are positive regulators of PRKN-independent mitophagy. Nature Communications, 12, 6101.

Publication 2021

Lysotracker Red DND-99 QVD-OPh SML0063) sotrastaurin Bradford reagent dye Complete EDTA-free protease inhibitors phosphatase inhibitors

Antimycin a Bafilomycin a1 Cayman Cccp Dithiothreitol Edta Enzastaurin Inhibitors Lysotracker Oligomycin Phosphatase Protease inhibitors Qvd oph Red dnd 99 Sotrastaurin Vps34 in1

Corresponding Organization : University of Oslo

Other organizations : Akershus University Hospital

Top 5 similar protocols

Variable analysis

independent variables

Lysotracker Red DND-99 (L7528)
Antimycin A (A8674)
DFP (379409)
DMOG (D3695)
SGC-GAK-1 (GAKi, SML2202)
SGC-GAK-1N (GAKc, SML2203)
QVD-OPh (SML0063)
Bafilomycin A1 (BML-CM110)
CCCP (BML-CM124)
Enzastaurin (S1055)
Oligomycin A (S1478)
Sotrastaurin (S2791)
MRT68921 (1190379-70-4)
VPS34-IN1 (1383716-33-3)
IVAP1966 (12 g)
IVAP1966 (12i)
HY-19764

dependent variables

Measured outcomes

control variables

Bradford reagent dye (#5000006)
1,4-dithiothreitol (DTT, #441496P)
Complete EDTA-free protease inhibitors (#05056489001)
Phosphatase inhibitors (#04906837001)

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