Cucumber Root-Knot Nematode Infection

Cucumber (Cucumis sativus L.) cultivar “Xintaimici” was used in this study. Seeds were surface sterilized using 4% sodium hypochlorite and germinated on moistened filter paper in darkness for the RHI and the PCI methods. For the SHI method, seeds were surface sterilized and germinated on MS solid medium. M. incognita race 2 was maintained on cucumber in sterilized soil. The egg masses were collected and sterilized with 0.5% sodium hypochlorite for 3 min and then submerged in sterile water at 25 ℃ for 3 days. Freshly hatched pre-J2s were collected using a 500-mesh screen and stored in 4 ℃ before infection.
R. rhizogenes strain K599 (Weidi Biotechnology, China) was used in this study to induce hairy roots which harboring plasmid pRi2659 (agropine type) and had a wide range of hosts including Cucurbitaceae.

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Zhang X., Li S., Li X., Song M., Ma S., Tian Y, & Gao L. (2023). Peat-based hairy root transformation using Rhizobium rhizogenes as a rapid and efficient tool for easily exploring potential genes related to root-knot nematode parasitism and host response. Plant Methods, 19, 22.

Publication 2023

Agropine Cucumber Cucumis sativus Cucurbitaceae Darkness Hairy Hosts range Infection Plasmid Roots Seeds Sodium hypochlorite Sterile Strain

Corresponding Organization :

Other organizations : China Agricultural University

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Variable analysis

independent variables

Cucumber (Cucumis sativus L.) cultivar "Xintaimici"
R. rhizogenes strain K599 (Weidi Biotechnology, China)

dependent variables

Not explicitly mentioned

control variables

Cucumber seeds were surface sterilized using 4% sodium hypochlorite
Cucumber seeds were germinated on moistened filter paper in darkness for the RHI and the PCI methods
Cucumber seeds were germinated on MS solid medium for the SHI method
M. incognita race 2 was maintained on cucumber in sterilized soil
Egg masses were collected and sterilized with 0.5% sodium hypochlorite for 3 min and then submerged in sterile water at 25 °C for 3 days
Freshly hatched pre-J2s were collected using a 500-mesh screen and stored in 4 °C before infection

positive control

Not explicitly mentioned

negative control

Not explicitly mentioned

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