Enzymatic Characterization of Marmoricola sp.

To determine hydrolysis of starch, urea, Tween 20, 40, 60, and 80, the isolate was cultured on TSA at 30°C for a week, as described by Cowan and Steel [37 ]. The enzyme activity was evaluated using API ZYM kit, API 20NE kit (bioMérieux, France), and acid production was tested API 50CH test (bioMérieux, France) according to the manufacturer’s instructions at 30°C for 2 days. The type strains, M. bovistercoris NEAU-LLE^T and M. pseudoresistence CC-5209^T, which are related to KUDC0405^T, were analyzed under the same conditions. The cell wall peptidoglycan was analyzed using an amino acid analyzer (L-8900; Hitachi, Japan). To analyze the polar lipids, two-dimensional thin layer chromatography (TLC) analysis were used according to Minnikin et al. [38 (link)]. The fatty acid composition analysis was performed using the Microbial Identification System from cells of the strain KUDC0405^T, and reference strains were incubated on TSA at 30°C for 7 days. To determine siderophore production by strain KUDC0405^T, chrome azurol S (CAS) media were used as previously described [39 (link), 40 (link)].