Measuring Endothelial Barrier Integrity

The cellular barrier properties were assessed by measuring the resistance values across confluent HPMECs using Millicell‐ERS (MERS00002, Millipore; Monaghan‐Benson & Wittchen, 2011 (link)). The 12‐well Transwell system was inserted into the 12‐well plate. HPMECs were plated in Transwell chambers. The electrode tips were inserted into the upper and lower compartments, and the resistance (R sample) was measured. The value for the blank well represented the blank resistance (R blank). The transendothelial electrical resistance (TEER) value was calculated based on the resistance per unit area as (R sample − R blank) × 1.33 cm (the area of a 12‐well Transwell chamber is 1.33 cm²; Tominaga et al., 2015 (link)).

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Kuang X., Wang Y., Liu S., Chang L., Yin Y., Li Z., Liu Y., Li W., Hou Y., Wang H., Liang J, & Jia Z. (2022). Tongxinluo enhances the effect of atorvastatin on the treatment of atherosclerosis with chronic obstructive pulmonary disease by maintaining the pulmonary microvascular barrier. Food Science & Nutrition, 11(1), 390-407.

Publication 2022

Millicell‐ERS (MERS00002,

Cellular Electrical resistance

Corresponding Organization : Hebei Yiling Hospital

Other organizations : State Administration of Traditional Chinese Medicine of the People's Republic of China, Hebei Medical University

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Variable analysis

independent variables

The researchers do not explicitly mention any independent variables that were manipulated in the study.

dependent variables

Transendothelial electrical resistance (TEER) value

control variables

The 12‐well Transwell system was inserted into the 12‐well plate.
HPMECs were plated in Transwell chambers.
The electrode tips were inserted into the upper and lower compartments.

controls

The value for the blank well represented the blank resistance (R blank).
No negative control is explicitly mentioned.

Annotations

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