Immunohistochemistry for caspase-3 was performed according to previously described methods [15 (link),16 (link)]. The sections were incubated overnight with caspase-3 antibody (1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA). The next day, the sections were incubated with biotinylated mouse secondary antibody (1:200; Vector Laboratories) for 1 hour. The secondary antibody was amplified with a Vector Elite ABC kit (1:100; Vector Laboratories). Antibody-biotin-avidin-peroxidase complexes were visualized using 0.03% DAB. The sections were mounted onto gelatin-coated slides, and the coverslips were mounted using Permount (Fisher Scientific).
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Caspase-3 Immunohistochemistry Protocol
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Other organizations : International St. Mary's Hospital, Catholic Kwandong University, Kyung Hee University, Kyung Hee University Hospital at Gangdong, Kyung Hee University Dental Hospital
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Variable analysis
independent variables
- Incubation of sections with caspase-3 antibody (1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA)
- Antibody-biotin-avidin-peroxidase complexes visualized using 0.03% DAB
- Previously described methods [15, 16]
- Incubation of sections with biotinylated mouse secondary antibody (1:200; Vector Laboratories) for 1 hour
- Amplification of secondary antibody with a Vector Elite ABC kit (1:100; Vector Laboratories)
- Mounting of sections onto gelatin-coated slides
- Coverslips mounted using Permount (Fisher Scientific)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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