High-performance liquid chromatography with diode array detectors (HPLC-PDA) was performed as described in previous work by Kazlauskaite et al. [36 (link)]. It was carried out using the Shimadzu Nexera X2 LC-30AD HPLC system (Shimadzu, Tokyo, Japan), equipped with an SPD-M20A diode array detector (DAD).
For the determination of polyphenols in plant extracts, an ACE 5 C18 250 × 4.6 mm column (Advanced Chromatography Technologies, Aberdeen, Scotland) was used. The mobile phase consisted of solvent A (acetic acid/methanol/deionised water) (1:10:89 v/v/v) and solvent B (acetic acid/methanol) (1:99 v/v). The linear gradient elution profile was as follows: 80% A/20% B at 0 min, 30% A/70% B at 30 min, and 90% A/10% B at 39 to 40 min. The flow rate was 1 mL/min, and the injection volume was 10 μL. Absorption was measured at 260 nm. Quantification of compounds was performed using reference standards.
For the determination of polyphenols in plant extracts, an ACE 5 C18 250 × 4.6 mm column (Advanced Chromatography Technologies, Aberdeen, Scotland) was used. The mobile phase consisted of solvent A (acetic acid/methanol/deionised water) (1:10:89 v/v/v) and solvent B (acetic acid/methanol) (1:99 v/v). The linear gradient elution profile was as follows: 80% A/20% B at 0 min, 30% A/70% B at 30 min, and 90% A/10% B at 39 to 40 min. The flow rate was 1 mL/min, and the injection volume was 10 μL. Absorption was measured at 260 nm. Quantification of compounds was performed using reference standards.
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