Immunohistochemical Analysis of E-cadherin and Vimentin

Tumor tissue sections were treated with 1% H₂O₂, blocked with 1% FBS (Gibco, CA, USA), and immunostained with anti-E-cadherin (1:1000; Cell Signaling Technology, MA, USA) or anti-Vimentin (1:500; Sigma-Aldrich, MO, USA) for 12 h at 4°C. After that, tumor tissue sections were incubated with a secondary antibody (1:500; Cell Signaling Technology) for 2 h at 25°C. All sections were developed by 3,3’-diaminobenzidine (DAB) kit (Thermo Scientific, CA, USA) [36 (link)].

Free full text: Click here

Liang J.H., Xu Q.D, & Gu S.G. (2022). LncRNA RSU1P2-microRNA let-7a-Testis-Expressed Protein 10 axis modulates tumorigenesis and cancer stem cell-like properties in liver cancer. Bioengineered, 13(2), 4285-4300.

Publication 2022

FBS anti-E-cadherin anti-Vimentin 3,3’-diaminobenzidine (DAB) kit

Antibody Cadherin 1 H2o2 Tissue Tumor Vimentin

Corresponding Organization : Cancer Hospital of Shantou University Medical College

Other organizations : First Affiliated Hospital of Shantou University Medical College

Top 5 similar protocols

Variable analysis

independent variables

Treatment of tumor tissue sections with 1% H2O2

dependent variables

Expression of E-cadherin
Expression of Vimentin

control variables

Blocking of tumor tissue sections with 1% FBS (Gibco, CA, USA)
Incubation of tumor tissue sections with primary antibodies (anti-E-cadherin and anti-Vimentin) for 12 h at 4°C
Incubation of tumor tissue sections with secondary antibody (1:500; Cell Signaling Technology) for 2 h at 25°C
Development of all sections using 3,3'-diaminobenzidine (DAB) kit (Thermo Scientific, CA, USA)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!