Immunohistochemical Analysis of Kidney Tissues

Kidney tissues were snap frozen embedded in OCT. Immunohistochemistry was performed as previously described [33 (link)]. Photomicrographs (400× or 200×) were taken using an Olympus BX41 microscope (Center Valley, PA). The following primary antibodies were used: anti-CD4, anti-CD8 (both from eBioscience, San Diego, CA), and anti-insulin (Dako North America, Carpinteria, CA). Immunohistochemistry was performed as well on formalin-fixed, paraffin-embedded tissue sections related to the graft. Photomicrographs (original magnification 40×) were taken using an Olympus BX41 microscope (Center Valley, PA). The antibodies used were Mouse anti-CD31 (PECAM-1) (Life Technologies, Carlsbad, CA, USA). Histology was evaluated by an expert pathologist.

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Ben Nasr M., Vergani A., Avruch J., Liu L., Kefaloyianni E., D’Addio F., Tezza S., Corradi D., Bassi R., Valderrama-Vasquez A., Usuelli V., Kim J., Azzi J., Essawy B.E., Markmann J., Abdi R, & Fiorina P. (2015). Co-transplantation of autologous MSCs delays islet allograft rejection and generates a local immunoprivileged site. Acta diabetologica, 52(5), 917-927.

Publication 2015

BX41 microscope anti-CD4 anti-CD8 anti-insulin

Antibodies Formalin Frozen Graft Immunohistochemistry Insulin Kidney Microscope Mouse Paraffin Pathologist Pecam 1 Photomicrographs Tissue

Corresponding Organization :

Other organizations : Boston Children's Hospital, Harvard University, Vita-Salute San Raffaele University, Brigham and Women's Hospital, University of Parma, Al-Azhar University, IRCCS Ospedale San Raffaele

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Variable analysis

independent variables

Tissue processing method (snap freezing and embedding in OCT)

dependent variables

Presence and localization of CD4, CD8, and insulin proteins (measured by immunohistochemistry)
Presence and localization of CD31 protein (measured by immunohistochemistry)

control variables

Microscope used (Olympus BX41)
Magnification used (400×, 200×, 40×)

positive controls

None specified

negative controls

None specified

Annotations

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