Cytochrome C and lysozyme were purchased from Sigma. Cross-linking of these proteins followed the same procedure as described previously.30 (link)Cross-linked peptides were analyzed by LC MSn utilizing an LTQ-Orbitrap XL MS (Thermo Fisher, San Jose, CA) coupled on-line with an Easy-nLC 1000 (Thermo Fisher, San Jose, CA) as previously described.3 (link),7 (link) Each MSn experiment consists of one MS scan in FT mode (350–1400 m/z, resolution of 60,000 at m/z 400) followed by two data-dependent MS2 scans in FT mode (resolution of 7500) with normalized collision energy at 20% on the top two MS peaks with charges at 3+ or up, and three MS3 scans in the LTQ with normalized collision energy at 35% on the top three peaks from each MS2.
Protocol detail
Protein Cross-linking and Mass Spectrometry
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Irvine Valley College
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- Cross-linking of cytochrome C and lysozyme proteins
- Analysis of cross-linked peptides by LC MS^n
- The cross-linking procedure followed the same protocol as described previously in reference 30.
- No positive or negative controls were explicitly mentioned in the provided information.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!