Genomic DNAs and total RNAs were isolated from ‘4-94-1 MR’ and ‘120 MOR’, ‘129 MOR’ and ‘129 MOR-MOR1’ petals at developmental stage 3 using a DNeasy Plant Mini Kit and a RNeasy Plant Mini Kit (Qiagen), respectively. First-strand cDNA was synthesized from 1 μg total RNA using oligo(dT)s and PrimeScript reverse transcriptase (Takara Bio). CHI (Dca60978 and Dca60979) cDNA fragments were obtained using specific primer sets (Dca60978 Fwd and Dca60978 Rv for Dca60978, Dca60979 Fwd and Dca60979 Rv for Dca60979) based on the nucleotide sequences of the cultivar ‘Francesco’ in the database Carnation DB (Yagi et al. 2014 (link)) (Supplemental Table 1). PCR was performed using Prime Star GXL polymerase (Takara Bio) under the following conditions: 2 min denaturation at 94°C, then 35 cycles of 10 s denaturation at 98°C, 15 s annealing at 55°C, 20 s extension at 68°C, and a final 1 min extension at 68°C. After an adenylation reaction, the amplified DNAs were introduced into the T-vector pMD20 (Takara Bio). The sequences of the genomic and cDNA fragments were determined using a BigDye terminator ver.3.1 cycle sequencing kit and an ABI PRISM 3130xl (Applied Biosystems). Nucleotide structures of Dca60978 and Dca60979, without the transposable element dTdic1, were confirmed by genomic and cDNA sequencing. Dca60979 with the dTdic1 insert was identified by its genomic sequence.