Molecular Regulators of Cortactin Signaling

The cortactin (ab33333), PTP1B EP1837Y (ab52650) and PTP1B EP1841Y (ab75856) antibodies were obtained from Abcam (Cambridge, MA). The Tks5 (sc30122) antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and labeled with Alexa Fluor 568 dye (Molecular Probes, Eugene, OR) for pY421-cortactin staining experiments as per the manufacturer’s instructions. The phospho-Y421 cortactin (C0739) antibody was from Sigma-Aldrich (St. Louis, MO). The PTP1B inhibitor (539741) was from Calbiochem (San Diego, CA). All secondary antibodies were Alexa Fluor conjugated and obtained from Molecular Probes (Life Technologies, Carlsbad, CA). The Pan-Mena antibody was purchased from Novus Biologicals (Littleton, CO; Cat# NBP1–87914). The antibody specific for Mena^INV used in this study has been described elsewhere59 . pEGFP-PTP1B C3 was a gift from Anna Huttenlocher (University of Wisconsin, Madison, WI). pCAX-eGFP and pCAX-eGFP-Mena^INV vectors were produced by the Gertler lab (MIT, Cambridge, MA)45 (link).

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Weidmann M.D., Surve C.R., Eddy R.J., Chen X., Gertler F.B., Sharma V.P, & Condeelis J.S. (2016). MenaINV dysregulates cortactin phosphorylation to promote invadopodium maturation. Scientific Reports, 6, 36142.

Publication 2016

ab75856 Alexa Fluor 568 dye

Alexa 568 Antibodies Antibody Biologicals Cortactin Molecular probes Novus Ptp1b Vectors

Corresponding Organization :

Other organizations : Albert Einstein College of Medicine, Massachusetts Institute of Technology

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Variable analysis

independent variables

Cortactin (ab33333) antibody
PTP1B EP1837Y (ab52650) antibody
PTP1B EP1841Y (ab75856) antibody
Tks5 (sc30122) antibody labeled with Alexa Fluor 568 dye
Phospho-Y421 cortactin (C0739) antibody
PTP1B inhibitor (539741)
PEGFP-PTP1B C3
PCAX-eGFP
PCAX-eGFP-Mena^INV

dependent variables

PY421-cortactin staining

control variables

All secondary antibodies were Alexa Fluor conjugated and obtained from Molecular Probes (Life Technologies, Carlsbad, CA)
The Pan-Mena antibody was purchased from Novus Biologicals (Littleton, CO; Cat# NBP1–87914)
The antibody specific for Mena^INV used in this study has been described elsewhere59 (no link found)

controls

Positive controls: None specified
Negative controls: None specified

Annotations

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