Blood Sample Collection and Preservation for Genetic Analysis

About 125 μl of blood were collected from the middle ear vein and applied onto the classic Whatman FTA® cards (Whatman Bioscience, Cambridge, UK) avoiding cross contamination of the four samples on each card [29 , 30 (link)]. The samples were then allowed to air-dry, labelled with cattle tag number, treatment regimen, sampling number, village name, parish, sub County, County and date of collection. They were packed in foil pouches with a silica gel desiccant (Sigma Aldrich, Co., Life sciences, USA) prior to shipping to the University of Edinburgh, UK for analysis.

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Muhanguzi D., Picozzi K., Hatendorf J., Thrusfield M., Welburn S.C., Kabasa J.D, & Waiswa C. (2014). Collateral benefits of restricted insecticide application for control of African trypanosomiasis on Theileria parva in cattle: a randomized controlled trial. Parasites & Vectors, 7, 432.

Publication 2014

silica gel desiccant

Blood Cattle Desiccant Middle ear Silica gel Treatment regimen Vein

Corresponding Organization :

Other organizations : Makerere University, University of Edinburgh, University of Basel

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Variable analysis

independent variables

Treatment regimen

dependent variables

Concentration of biomarkers in the blood samples

control variables

Amount of blood collected (125 μl)
Use of Whatman FTA® cards to collect and store the samples
Drying of the samples
Labelling of the samples with relevant information
Packaging of the samples in foil pouches with silica gel desiccant
Shipping of the samples to the University of Edinburgh, UK for analysis

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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