This test demonstrates the ability of certain bacteria to decompose the amino acid tryptophan to indole, which accumulates in the medium. Isolated colony of the test organism was emulsified in tryptophan broth (peptone water) and incubated at 37°C for 24 hours in ambient air. 0.5 ml of Kovac's reagent was added to the broth culture down the side of the tube and observed for color change at meniscus. The development of a -red color (benzaldehyde reagents) within 20 seconds indicates the presence of indole. A negative test is colorless or slightly yellow.
Protocol detail
Tryptophan Decomposition Assay
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : University of Port Harcourt, Rivers State University, Federal College of Education, Kano
Top 5 similar protocols
Variable analysis
independent variables
- Isolated colony of the test organism
- Accumulation of indole in the medium
- Tryptophan broth (peptone water)
- Incubation at 37°C for 24 hours in ambient air
- Addition of 0.5 ml of Kovac's reagent to the broth culture
- Positive control: Development of a red color (benzaldehyde reagents) within 20 seconds indicates the presence of indole.
- Negative control: Colorless or slightly yellow color indicates a negative test.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!