Protocol for sensitization and challenge was based on the previous published method with minor modification (23 (link)). Briefly, mice were injected intraperitoneally (i.p.) on days 0, 3, 5 with HDM extract containing 10 µg of Der p1 (Indoor Biotechnologies, VA, USA) in 200 µL of sterile saline and then challenged intranasally (i.n.) with the same agent on days 10, 12 14, 16, and 17. Control mice were received phosphate-buffered saline (PBS) during the sensitization and challenge phases. Mice were sacrificed 24 hours after the last challenge for airway reactivity measurement.
Protocol detail
Murine Model of Allergic Airway Inflammation
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Mice
Phosphate
Saline
Sterile
Corresponding Organization : Chinese Academy of Medical Sciences & Peking Union Medical College
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Variable analysis
independent variables
- Injection of HDM extract containing 10 µg of Der p1 in 200 µL of sterile saline on days 0, 3, 5
- Intranasal challenge with the same HDM extract on days 10, 12, 14, 16, and 17
- Airway reactivity measurement
- Phosphate-buffered saline (PBS) administered during the sensitization and challenge phases in control mice
- Positive control: None mentioned
- Negative control: Control mice received PBS during the sensitization and challenge phases
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