We collected clinical data, such as weight, height, waist circumference and other data. The BMI was calculated as weight (in kg) divided by the square of height (in m). Fasting plasma glucose, serum cholesterol, serum triglycerides, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol were measured as described in a previous protocol [16 (link)]. Glycated hemoglobin (HbA1c) was estimated by high performance liquid chromatography using a Variant™ machine (Bio-Rad, Hercules, CA, USA). Serum insulin concentration was estimated using an enzyme-linked immunosorbent assay (Dako, Glostrup, Denmark). Total serum adiponectin was measured by radioimmunoassay.
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