To create the CP-TIB nerve graft, a freshly axotomized common peroneal (CP) nerve branch was grafted to the acutely or chronically denervated tibial (TIB). To mimic a delayed graft, the TIB nerve branch was transected 2–5 mm distal to the sciatic trifurcation and sutured to the quadriceps muscle. After 8 weeks of delay7 (link), the proximal (CP) and distal (TIB) stumps were aligned and sutured into either end of a 5 mm silicone conduit (Tuzic, Siliclear tubing, 4.6 mm OD, 1.98 mm ID) with 10/0 ethilon sutures to produce the CP-TIB graft with an interposed silicone conduit for cell and protein isolation. Immediate repair was achieved similarly but with immediate CP-TIB cross-suture (graft).
Delayed Nerve Graft with Silicone Conduit
To create the CP-TIB nerve graft, a freshly axotomized common peroneal (CP) nerve branch was grafted to the acutely or chronically denervated tibial (TIB). To mimic a delayed graft, the TIB nerve branch was transected 2–5 mm distal to the sciatic trifurcation and sutured to the quadriceps muscle. After 8 weeks of delay7 (link), the proximal (CP) and distal (TIB) stumps were aligned and sutured into either end of a 5 mm silicone conduit (Tuzic, Siliclear tubing, 4.6 mm OD, 1.98 mm ID) with 10/0 ethilon sutures to produce the CP-TIB graft with an interposed silicone conduit for cell and protein isolation. Immediate repair was achieved similarly but with immediate CP-TIB cross-suture (graft).
Corresponding Organization :
Other organizations : New York State College of Veterinary Medicine, Cornell University, Parker Hannifin (United States), Hospital for Sick Children, Indiana University Bloomington
Variable analysis
- Timing of nerve graft (immediate vs. delayed)
- Outcomes of nerve graft procedures (not explicitly specified)
- Anesthesia protocol (3% isoflurane for induction, 2% isoflurane for maintenance, and oxygen)
- Analgesia (subcutaneous buprenorphine simbadol 0.1 mg/kg pre-operatively and 24 h after surgery)
- Surgical technique (sterile microsurgical with operating microscope)
- Nerve graft material (5 mm silicone conduit)
- Immediate CP-TIB nerve graft
- None mentioned
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