A container filled with saline solution was used to hatch the eggs of brine shrimp under suitable conditions of temperature, light, and aeration. The eggs hatched into nauplii (larval stage of brine shrimp) within a day. A series of test solutions with different concentrations of the sample were prepared by diluting them in the solvent. 10 brine shrimp nauplii were added to each container, including the control using a micropipette. The nauplii are allowed to swim freely in the test solutions. The containers were incubated under suitable conditions for 24 hours. The temperature and lighting conditions were maintained. After the incubation period, the containers were examined to determine the number of surviving brine shrimp nauplii in each concentration and control. The lethality or mortality rate is calculated by counting the number of dead nauplii (5, 18, 19) .