Paraffin-embedded Sections (2 μm) of kidney were stained with periodic acid-Schiff (PAS), and Sirius red by routine protocol [19 (link)]. Immunohistochemistry was performed by routine method [20 (link)]. Primary antibodies include wnt3a (Abcam, ab28472), active-β-catenin (Millipore, 05-665), DKK1 (Abcam, 109416), AGT (Abcam, ab213705), ACE-1 (Abcam, ab222739), AT1 (Abcam, ab18801).
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