We followed our transdifferentiation protocol described in detail elsewhere [47 (link)] and in supplementary materials and methods. Pictures of cells were taken using a Nikon Eclipse Ti-S/L 100 inverted microscope equipped with a CoolSNAP Myo, 20 MHz, 2.8 Megapixel, 4.54 × 4.54 µm pixels camera and with a Nikon CFI Super fluor 20X DIC prism objective. Further information about the protocols we followed for collecting pictures and tracing cells can be found in supplementary materials and methods.
Treatments with colchicine (Sigma–Aldrich, C9754) and dopamine (Sigma-Aldrich, H8502-259), involved three concentrations for colchicine (0.4 µM, 0.5 µM and 0.75 µM) and two for dopamine (4 mM and 5 mM). Detail information about dopamine preparation as well as preincubations with a D1-like receptor antagonist is provided in supplementary materials and methods. Tracing and cell characterization were done blinded.
Human neurons were obtained from ScienCell Research laboratories (1520-10) and cultured following the manufacturer’s instructions. Further information about the procedures followed using human neurons is listed in Supplementary Materials and Methods.
Treatments with colchicine (Sigma–Aldrich, C9754) and dopamine (Sigma-Aldrich, H8502-259), involved three concentrations for colchicine (0.4 µM, 0.5 µM and 0.75 µM) and two for dopamine (4 mM and 5 mM). Detail information about dopamine preparation as well as preincubations with a D1-like receptor antagonist is provided in supplementary materials and methods. Tracing and cell characterization were done blinded.
Human neurons were obtained from ScienCell Research laboratories (1520-10) and cultured following the manufacturer’s instructions. Further information about the procedures followed using human neurons is listed in Supplementary Materials and Methods.
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