Comprehensive Nanoparticle Characterization for Pharmaceutical Applications

The mean particle size and polydispersity index were assessed using dynamic light scattering (DLS, Zetasizer Nano ZS, Malvern Instruments Ltd., Worcestershire, UK). The electrical characteristics of the samples were determined using electrophoretic light scattering. Transmission electron microscopy (TEM, Hitachi High-Tech Corporation, Tokyo, Japan) was employed to observe the morphological characteristics of the NLC-Pip–BSA samples. Spectral characterization was achieved by Fourier-transform infrared spectroscopy (FTIR, Bruker Vertex 70, Etilingen, Germany) and fluorescence spectroscopy (FP-650 Spectrofluorometer Jasco, Tokyo, Japan). The entrapment efficiency was determined by UV-Vis spectroscopy (UV-Vis Spectrophotometer V670 Jasco, Tokyo, Japan) [31 (link),32 (link)]. In vitro activity of the prepared NLCs was studied using real-time cells assay (RTCA, ACEA Biosciences, San Diego, CA, USA), MTS assay (CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, WI, USA), and flow cytometry(FACS Canto II cytometers, Becton Dickinson, Immunocytometry System, Mountain View, CA, USA) [33 (link),34 (link),35 (link),36 (link)]. For detailed characterization procedures see Supplementary Materials Section, S1.2.1. to S1.2.10.

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Tincu R., Mihaila M., Bostan M., Teodorescu F., Istrati D., Badea N, & Lacatusu I. (2023). Novel Bovine Serum Albumin-Decorated–Nanostructured Lipid Carriers Able to Modulate Apoptosis and Cell-Cycle Response in Ovarian, Breast, and Colon Tumoral Cells. Pharmaceutics, 15(4), 1125.

Publication 2023

Zetasizer Nano ZS Vertex 70 RTCA CellTiter 96 Aqueous One Solution Cell Proliferation Assay

Assay Cell proliferation Cells Electrical Electrophoretic Flow cytometry Fluorescence spectroscopy Ftir Light Promega Rtca Spectroscopy Transmission electron microscopy

Corresponding Organization : Titu Maiorescu University

Other organizations : Romanian Academy, Institutul National Victor Babes

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Variable analysis

independent variables

Concentration of NLC-Pip–BSA samples

dependent variables

Mean particle size
Polydispersity index
Electrical characteristics
Morphological characteristics
Spectral characteristics
Entrapment efficiency
In vitro activity (cell viability, proliferation, and apoptosis)

control variables

Dynamic light scattering (DLS, Zetasizer Nano ZS, Malvern Instruments Ltd., Worcestershire, UK)
Electrophoretic light scattering
Transmission electron microscopy (TEM, Hitachi High-Tech Corporation, Tokyo, Japan)
Fourier-transform infrared spectroscopy (FTIR, Bruker Vertex 70, Etilingen, Germany)
Fluorescence spectroscopy (FP-650 Spectrofluorometer Jasco, Tokyo, Japan)
UV-Vis spectroscopy (UV-Vis Spectrophotometer V670 Jasco, Tokyo, Japan)
Real-time cells assay (RTCA, ACEA Biosciences, San Diego, CA, USA)
MTS assay (CellTiter 96 Aqueous One Solution Cell Proliferation Assay, Promega, Madison, WI, USA)
Flow cytometry (FACS Canto II cytometers, Becton Dickinson, Immunocytometry System, Mountain View, CA, USA)

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