At the beginning and end of the study, 10 cc of venous blood samples were taken and after separation of serum, biochemical parameters of glycemic status including fasting blood glucose (FBG), 2-hour Post Prandial (2hpp), (2-hour after breakfast), and HbA1c%, insulin and CRP measured in the plasma of the participants. Tubes with and without EDTA were used to collect blood samples. Tubes without EDTA were centrifuged (Beckman Avanti J-25, USA, 3000 rpm,10 min) and finally maintained at minus − 70 ° C for final measurements. FBG concentration was measured by the enzymatic method using an Abbot ModelAclyon 300, USA auto analyzer with Pars-Azmone kit (Tehran, Iran). The percentage of HbA1c was determined by high performance liquid chromatography (HPLC). Plasma insulin was measured by using a chemiluminescent immunoassay method (LIAISON analyzer (310,360) Diasorin S.P.A., Verecelli, Italy). Insulin resistance (IR) was calculated according to the following formula: Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) (µU/ml) = (Fasting insulin (U/ml) × FBG (mg/dl)/405) [18 (link)]. CRP concentration was measured by using an immune turbid metric assay (Pars Azmoon kit. Iran).
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