Quantifying Plant Protein and GST Activity

Total plant protein was extracted in native condition as described previously [45 (link)] and quantified using Bradford method [46 (link)]. The glutathione S-transferase (GST, EC 2.5.1.18) enzyme activity was measured spectrophotometrically using reduced glutathione and 1-chloro-2,4-dinitrobenzene (CDNB) substrates as published earlier [24 (link)]. The specific activity of GST (nmol/min/mg protein) was calculated and compared amongst the samples. This experiment was performed in triplicates and data was represent as the average value ± standard deviation (n = 3).

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Islam S., Rahman I.A., Islam T, & Ghosh A. (2017). Genome-wide identification and expression analysis of glutathione S-transferase gene family in tomato: Gaining an insight to their physiological and stress-specific roles. PLoS ONE, 12(11), e0187504.

Publication 2017

Dinitrobenzene Ec 18 Enzyme activity Glutathione s transferase Plant protein Protein Reduced glutathione

Corresponding Organization : Shahjalal University of Science and Technology

Other organizations : University of Dhaka

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Total plant protein extracted in native condition
Glutathione S-transferase (GST, EC 2.5.1.18) enzyme activity

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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