Western blotting assays were performed by the protocol described in our previous report [7 (link)]. The following antibodies are used for immune blotting assays: monoclonal anti-Flag (M185-3L, MBL), Akt (610860, BD Biosciences), β-Actin (sc-4778, Santa Cruz) and the cell signaling antibodies (p-Akt, ERK, and p-ERK). GNPAT antibody was purchased from Abcam (ab75060).
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