Normal prostate epithelial cell (RWPE1) and PCa cells (DU145, LNCaP, PC-3 and C42B) were bought from American Type Culture Collection (ATCC; Manassas, VA, USA). Cells were cultured in line with previous description [15 (link)]. They were cultured with 10% FBS and 1% antibiotics in DMEM (Gibco, Rockville, MD, USA). In order to activate the Wnt/β-catenin signaling pathway, DU145 cells were treated with lithium chloride (LiCl; Sigma-Aldrich, St. Louis, MO, USA) for 24 h.
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