The DAVID database was utilized to identify essential genes in the negative regulation of the apoptotic process. The findings were examined using the GeneMANIA database to identify the most significant genes in anti-apoptotic activities such as apoptotic signaling pathway negative regulation, intrinsic apoptotic signaling pathway negative regulation, and endothelial cell apoptotic process negative regulation. The Enrichr database was utilized to verify the acquired findings.
In this regard, the sequences of NFE2L2 and SIRT1 genes were collected from NCBI nucleotide [48 ]. To design siRNAs, Eurofins Genomic’s siRNA design tool was used, which is an online gadget providing the use of guidelines initially provided by Tuschl et al. [49 (link)]. In addition, it applies the scorings criteria [12 (link)] (including target site, length of siRNA, specificity checking, and nucleotide content of siRNA) developed by Reynolds et al. [50 (link)], Ui-Tei et al. [51 (link)], and Vert et al. [52 (link)]. To ensure the safety and prevent off-target effects of the designed siRNAs, the sequences were aligned by the human genome utilizing align sequences nucleotide BLAST [53 ].
In this regard, the sequences of NFE2L2 and SIRT1 genes were collected from NCBI nucleotide [48 ]. To design siRNAs, Eurofins Genomic’s siRNA design tool was used, which is an online gadget providing the use of guidelines initially provided by Tuschl et al. [49 (link)]. In addition, it applies the scorings criteria [12 (link)] (including target site, length of siRNA, specificity checking, and nucleotide content of siRNA) developed by Reynolds et al. [50 (link)], Ui-Tei et al. [51 (link)], and Vert et al. [52 (link)]. To ensure the safety and prevent off-target effects of the designed siRNAs, the sequences were aligned by the human genome utilizing align sequences nucleotide BLAST [53 ].
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