Paper Spray Mass Spectrometry of Angiotensin I

Example 11

FIG. 15 is a paper spray mass spectrum of angiotensin I solution (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu (SEQ ID NO: 1), 10 μL, 8 μg/mL in methanol/water, 1:1, v/v) on chromatography paper (spray voltage, 4.5 kV). The inset shows an expanded view over the mass range 630-700. The protonated ([M+2H]²⁺) and sodium-adduct ions ([M+H+Na]²⁺, [M+2Na]²⁺) are the major ionic species.

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US11867684B2. Sample dispenser including an internal standard and methods of use thereof (2024-01-09). Purdue Research Foundation [US]. Inventors: Zheng Ouyang [US], He Wang [US], Nicholas E. Manicke [US], Robert Graham Cooks [US], Qian Yang [US], Jiangjiang Liu [US].

Patent 2024

Angiotensin i Arg val Asp tyr Ionic Mass spectrum Methanol Paper chromatography Sodium

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Variable analysis

independent variables

Spray voltage

dependent variables

Mass spectrum of angiotensin I solution

control variables

Angiotensin I solution (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu (SEQ ID NO: 1), 10 μL, 8 μg/mL in methanol/water, 1:1, v/v)
Chromatography paper

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