Western Blot Analysis of Cleaved Caspase 3 and PARP-1

Total cell lysates were prepared and analyzed by Western blot as described previously [20 (link)]. We used the following primary antibodies—for the detection of cleaved caspase 3: monoclonal antibody (MAB10753) from MilliporeSigma, St. Louis, MO, USA) or monoclonal antibody (SC-271028) from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and for PARP-1: SC-56196 from Santa Cruz (specific for the cleaved form) and #9542 from Cell Signaling Technology (Danvers, MA, USA) (recognizing full-length and cleaved PARP-1). Horseradish peroxidase-antibody conjugates (i.e., secondary antibodies) were obtained from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA). All antibodies were used according to the suppliers’ recommendations. For detection, SuperSignal West Pico PLUS Chemiluminescent Substrate was used (ThermoFisher Scientific, Waltham, MA, USA). Immunoblots were repeated to confirm the results. The uncropped Western blot images can be found at Figure S7.