Assessing Mouse Visual Acuity using Virtual Optometry

Mouse visual acuity (VA) was assessed using the Virtual Optometry System (Optometry, Cerebral Mechanics, Inc., Canada). Briefly, the virtual rotating columns were projected on the LCD monitors with different spatial frequencies in cycles/degree (c/d). The mouse head movement in response to the rotating virtual columns was noted. The VA was defined as the highest spatial frequency the mouse was able to respond to the virtual rotating columns. The spatial frequency was changed starting from 0.1 c/d with a step size of 0.05 c/d until the mouse stopped responding. When VA ≤ 0.25 c/d, the mouse was considered to develop impaired VA, as defined by previous studies (26 (link), 27 (link), 34 (link), 35 (link)). VA was performed right before each in vivo MRI scan session.

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Yang H.C., Lavadi R.S., Sauerbeck A.D., Wallendorf M., Kummer T.T., Song S.K, & Lin T.H. (2023). Diffusion basis spectrum imaging detects subclinical traumatic optic neuropathy in a closed-head impact mouse model of traumatic brain injury. Frontiers in Neurology, 14, 1269817.

Publication 2023

Virtual Optometry System

Corresponding Organization :

Other organizations : Washington University in St. Louis

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Variable analysis

independent variables

Spatial frequency of the virtual rotating columns (cycles/degree)

dependent variables

Mouse head movement in response to the virtual rotating columns
Visual acuity (VA) defined as the highest spatial frequency the mouse was able to respond to the virtual rotating columns

control variables

The virtual rotating columns were projected on the LCD monitors
The spatial frequency was changed starting from 0.1 c/d with a step size of 0.05 c/d until the mouse stopped responding

controls

Previous studies (26, 27, 34, 35) have defined VA ≤ 0.25 c/d as impaired visual acuity in mice

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