Immunoblotting for Protein Expression

For protein expression analysis, a standard immunoblotting protocol was followed as described previously (10 (link)). The antibodies used in this study were: anti-GDF15 (Santa Cruz Biotechnology), anti-RhoB total (Abcam), anti-phospho (Thr202/Tyr204) Erk1/2 (Cell Signaling Technology), anti-total Erk1/2 (Cell Signaling Technology), anti-phospho-c-Jun (S63, Abcam), and anti-total-c-Jun (Abcam). Antibodies against β-actin or β-tubulin were used as loading control.

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Kalli M., Voutouri C., Minia A., Pliaka V., Fotis C., Alexopoulos L.G, & Stylianopoulos T. (2019). Mechanical Compression Regulates Brain Cancer Cell Migration Through MEK1/Erk1 Pathway Activation and GDF15 Expression. Frontiers in Oncology, 9, 992.

Publication 2019

anti-GDF15 anti-phospho (Thr202/Tyr204) Erk1/2 anti-total Erk1/2 anti-phospho-c-Jun

Actin Antibodies Erk1 Gdf15 Protein Tubulin

Corresponding Organization : University of Cyprus

Other organizations : National Technical University of Athens

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of GDF15, RhoB, phospho-Erk1/2, total Erk1/2, phospho-c-Jun, and total c-Jun

control variables

Antibodies against β-actin or β-tubulin used as loading controls

controls

Positive control: None mentioned
Negative control: None mentioned

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