Apoptosis Induction in Gastric Cancer Cells

SNU16 human stomach cancer cell line was purchased from KCLB (Seoul, Korea). To induce apoptosis, cells were treated with cisplatin (300 ng/ml), cetuximab (200 µg/ml), and cisplatin (300 ng/ml) plus cetuximab (200 µg/ml) in combination for 24 h. The concentrations of cisplatin and cetuximab were chosen according to the previous reports [22] (link), [23] (link). After treatment, cells were incubated with 10 µM of fluorescein isothiocyanate (FITC)-conjugated linear or cyclic form of ApoPep-1 at 4°C for 1 h. As control, cells were stained with Alexa488-conjugated annexin V (Life technologies, Carlsbad, CA) for 15 min at RT. Percentages of fluorescent (peptide-bound or annexin V-bound) cells were measured by flow cytometry.