Exo-MS was incubated with 1 mM fluorescent lipophilic tracer DiR (1, 1-octadecyl-3,3,3, 3-tetramethylindotricarbamine iodide) (Invitrogen, D12731, Life Technologies) at room temperature in the dark for 15 minutes. The exosomes were then separated at 120000g×70 min and the excess dye was washed. Exo-MS with a concentration of 100μg/100μL/mice was injected into the anesthetized mice via the tail vein. In vivo imaging was performed 24 h after injection, after which the mice were sacrificed and the relevant organs were removed. In the control group, 100ul PBS was injected into the tail vein and observed 24 hours later. DiR-EVs distribution was analyzed using an IVIS Spectrum system (Perkin Elmer).24 (link)
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In Vivo Tracking of Exosome Uptake
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Corresponding Organization :
Other organizations : Shandong First Medical University, Shandong Provincial Hospital, Qilu Hospital of Shandong University, Taian City Central Hospital, Qingdao University
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Variable analysis
independent variables
- Incubation of Exo-MS with 1 mM fluorescent lipophilic tracer DiR at room temperature in the dark for 15 minutes
- DiR-EVs distribution analyzed using an IVIS Spectrum system
- Injection of 100ul PBS into the tail vein and observation 24 hours later
- Positive control: Exo-MS with DiR tracer
- Negative control: Injection of PBS
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