Media that was pre incubated in the anaerobic chamber for 1 d was inoculated with equal ratios of AJB715 and LS25 at a final concentration of 1 × 103 CFU/ml of each strain. Final concentrations of 20 mM L-tartrate, 20 mM glycerol, and 0.2% casamino acids, as well as 40 mM sodium nitrate or 40 mM potassium tetrathionate were added as indicated. The strains were grown in the anaerobic chamber for 18 h at 37°C and recovery of each strain were determined by spread plating serial dilutions on selective LB agar plates.
Protocol detail
Anaerobic Co-culture Assay
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Corresponding Organization :
Other organizations : University of California, Davis, The University of Texas Southwestern Medical Center, Vanderbilt University Medical Center
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Variable analysis
independent variables
- Presence of 20 mM L-tartrate
- Presence of 20 mM glycerol
- Presence of 0.2% casamino acids
- Presence of 40 mM sodium nitrate
- Presence of 40 mM potassium tetrathionate
- Recovery of strain AJB715
- Recovery of strain LS25
- Inoculation ratio of AJB715 and LS25 (1:1)
- Initial concentration of each strain (1 × 10^3 CFU/ml)
- Incubation time (18 h)
- Incubation temperature (37°C)
- Anaerobic conditions
- Positive control: Not specified
- Negative control: Not specified
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