The antibodies 8B8, 68, AP3, AP5, LIBS1, LIBS6 have been previously described6 (link),22 (link),23 (link). Human embryonic kidney 293 (HEK293) cells were obtained from the American Tissue Type Collection (ATCC, Manassus, VA). CHOLec 3.2.8.1 cells were provided by Pamela Stanley24 (link). Truncated recombinant human vitronectin N-terminal fragment (VTN-N) was purchased from Thermo Fisher scientific (Waltham, MA). Cell culture media and antibiotics were prepared by the University of California, San Francisco Cell Culture Facility using deionized water and analytical grade reagents. Fetal calf serum was obtained from Invitrogen (Carlsbad, CA). The CHOlec 3.2.8.1 cell line was functionally verified by production of hypoglycosylated recombinant protein by SDS-PAGE. The cell line was not tested for mycoplasma contamination.
Protocol detail
Characterization of Antibodies and Cell Lines
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Antibiotics
Antibodies
Cell
Cell culture
Cell line
Culture media
Embryonic
Fetal calf serum
Human
Kidney
Mycoplasma
Recombinant protein
Sds page
Vitronectin
Corresponding Organization : Howard Hughes Medical Institute
Other organizations : Yale University
Top 5 similar protocols
Variable analysis
independent variables
- Antibodies: 8B8, 68, AP3, AP5, LIBS1, LIBS6
- Not explicitly mentioned
- HEK293 cells obtained from ATCC
- CHOLec 3.2.8.1 cells provided by Pamela Stanley
- Truncated recombinant human vitronectin N-terminal fragment (VTN-N) purchased from Thermo Fisher Scientific
- Cell culture media and antibiotics prepared by the University of California, San Francisco Cell Culture Facility
- Fetal calf serum obtained from Invitrogen
- The CHOlec 3.2.8.1 cell line was functionally verified by production of hypoglycosylated recombinant protein by SDS-PAGE
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!