Optimized Microcystis Genes in E. coli

MAE_06010 gene from Microcystis aeruginosa NIES-843 and MiAbW_01735 gene from Microcystis aeruginosa NIES-4325 were optimized according to the codon usage in E. coli and synthesized by GENEWIZ (Suzhou, China). The chaperone plasmids set (pG-KJE8, pGro7, pKJE7, pG-Tf2, pTf16) was from Takara Bio Inc. (Dalian, China). EasyPfu DNA polymerase, T4 DNA ligase, DNA marker, Fast Mutagenesis System and FlyCut endonucleases were obtained from TransGen Biotech (Beijing, China). SSADH was purified by our laboratory [35 (link),36 (link)]. The gene names used in this study were adopted from CyanoOmicsDB (http://www.cyanoomics.cn/lz/index, accessed on 25 September 2022) [22 (link)]. All other chemicals were purchased from Solarbio (Beijing, China) with the highest purity unless otherwise specified.