The stabilized NTS1 mutant with T4 lysozyme replacing most of intracellular loop 3 was expressed in insect cells and purified by cobalt affinity chromatography. It was crystallized in lipidic cubic phase. Diffraction data were collected at the GM/CA-CAT beamline of the Advanced Photon Source at the Argonne National Laboratory. The structure was solved by molecular replacement using data from a single crystal. Full Methods and any associated references are available in the online version of the paper at www.nature.com/nature.
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Other organizations :
National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases, MRC Laboratory of Molecular Biology, New York Structural Biology Center, National Institute of Neurological Disorders and Stroke
Stabilized NTS1 mutant with T4 lysozyme replacing most of intracellular loop 3
dependent variables
Crystal structure of the stabilized NTS1 mutant
control variables
Purification by cobalt affinity chromatography
Crystallization in lipidic cubic phase
Diffraction data collection at the GM/CA-CAT beamline of the Advanced Photon Source at the Argonne National Laboratory
controls
The structure was solved by molecular replacement using data from a single crystal
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